Intracellular Molecular Delivery

Delivering molecules into cells can be made more precise with vectors that direct intracellular cargo to particular compartments. Our work has focused on mitochondrial targeting with a versatile peptide based vector that promotes efficient cellular uptake and strong mitochondrial localization.

Microscopic view of cells

Mitochondrial Drug Delivery

Using a peptide-based delivery vector that can carry reactive cargo into the mitochondria of live mammalian cells, we have delivered clinically-utilized anticancer drugs that typically act within the nucleus. This approach has elucidated a variety of interesting activities for mitochondrially-localized probes. We’ve also shown that mitochondrial delivery can detoxify antimicrobials within human cells by sequestering the drugs within this organelle.

Infographic showing mitochondria-specific DNA damage and siRNA knockdown of DNA repair or replication factors

Mitochondrial Probes for the Discovery of New Organelle-Specific Activities

Mitochondrial probes also enable high-throughput screening for new organelle-specific factors. Using siRNA or CRISPR/Cas-based screening to knockdown factors in the nucleus that may function in mitochondria, synergistic effects can be detected in the presence of a mt-targeted probe. This type of analysis has allowed us to identify new factors that are key players in mitochondrial function.

Learn More About Our Research

The projects underway involve aspects of diverse disciplines!